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@#AIM: To investigate the clinical effect of vitamin A Palmitate Eye Gel combined with Tobramycin Eye Drops on corneal epithelial injury.<p>METHODS: Totally 80 patients(100 eyes)with corneal epithelial injury who came to our hospital from May 2015 to March 2016 were randomly divided into treatment group(50 eyes)and control group(50 eyes). The treatment group was treated with vitamin A Palmitate Eye Gel combined with Tobramycin Eye Drops, while the control group was treated with Tobramycin Eye Drops only. The curative effect of two groups was compared after 2wk and 4wk of treatment.<p>RESULTS: The cure rate was 80% and the effective rate was 94% in the treatment group. The cure rate was 70% and the effective rate was 78% in the control group. There was significant difference in the effective rate between the two groups(<i>P</i><0.05). The results of SⅠt and BUT test showed that the time of treatment group was significantly longer than that of control group(<i>P<</i>0.01). The score of symptoms and signs in the treatment group was significantly lower than that in the control group after 2wk of treatment(<i>P<</i>0.01), but there was no significant between treatment groups and control grouop after 4wk of treatment(<i>P></i>0.05).<p>CONCLUSION: Vitamin A Palmitate Eye Gel combined with Tobramycin Eye Drops can significantly improve dry eye symptoms and effectively shorten the healing time of corneal epithelium. It is an effective method to treat corneal epithelial injury.
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·AIM: To observe the efficacy of vitamin A palmitate eye gel and carboxymethylcellulose sodium eye drops on prevention of dry eye after phacoemulsification. ·METHODS: Ninety patients ( ninety eyes ) with age-related cataract enrolled in our hospital from March 2016 to August 2017 were performed phacoemulsification with intraocular lens implantation. They were randomly divided into three groups as control group ( n = 30 ), treated group Ⅰ ( n=30) and treated group Ⅱ ( n=30). The control group was administered with tobramycin and dexamethasone eye drops for 15d as the basis therapy. In treated group Ⅰ, patients were administered with carboxymethylcellulose sodium eye drops for 30d based on the treatment of the control group. The treated groupⅡ was administered with vitamin A palmitate eye gel for 30d on the basis of the treated group Ⅰ. The dry eye symptom score, corneal fluorescence ( FL ) staining scores, breakup time of tear film ( BUT) and Schirmer Ⅰtest ( SⅠt) without topical anesthesia were examined in 1d before operation and 15d and 30d after operation. · RESULTS: There were no statistically significant differences in subjective symptom scores of dry eye, BUT values, FL scores, and SⅠt values among the three groups before treatment (P>0. 05). After treatment, dry eye symptom scores, FL scores, and S Ⅰ t values increased at first and then decreased with time. BUT values decreased at first and then increased. Fifteen and thirty days after surgery, dry eye symptom scores, FL scores, and SⅠt values were significantly lower in the treated group Ⅰ and the treated group Ⅱ than in the control group. While BUT values were significantly higher than that in the control group. The dry eye symptom scores and S Ⅰ t value of treated group Ⅱ were significantly lower than the treated groupⅠ, and the BUT value was significantly higher than that of the treated group Ⅰ(P<0. 05). · CONCLUSION: Phacoemulsification combined with intraocular lens implantation has a certain damage of ocular surface tissue on the initial stage. The application of vitamin A palmitate eye gel combined carboxymethylcellulose sodium eye drops can improve the dry eye symptoms.
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· AIM:To investigate the effect of sodium hyaluronate eye drops combined with vitamin A palmitate ophthalmic gel on levels of tear film stability and inflammatory cytokines in patients with dry eye.· METHODS:A total of 100 patients with dry eye treated in our hospital from January 2015 to February 2017 were randomly divided into control group and observation group,50 cases in each group.Patients in the control group were treated with sodium hyaluronate eye drops.Patients in the observation group were given vitamin A palmitate ophthalmic gel on the basis of the control group,and then the clinical efficacy,tear film stability and the level of inflammatory cytokines were detected in the two groups.· RESULTS:After treatment,the levels of BUT and S I t in both groups increased significantly compared with that before treatment,and FL was significantly lower than that before treatment.The levels of BUT and S I t in the observation group after treatment were 11.24±0.22s and I1.4 ± 0.17mm/5min respectively,which was high than that of control groups(P＜0.05).FL level was 1.78±0.10 points,which was significantly lower than that of the control group after treatment (P＜0.05).After treatment,the levels of IL-1β and TNF-αsignificantly decreased in both groups.The levels of IL-1β and TNF-α in the observation group were 34.38± 5.58ng/L and 134.47 ± 12.14ng/L,significantly lower than those of the control group after treatment,the difference was statistically significant (P ＜ 0.05).The total effective rate of observation group after treatment was 91.7％,significantly higher than that of the control group after treatment,the difference was statistically significant (P＜ 0.05).· CONCLUSION:Sodium hyaluronate eye drops combined with vitamin A palmitate ophthalmic gel can relieve the symptoms of patients with dry eye effectively,increase the stability of tear film,and reduce the levels of inflammatory factors in tears,which is reliable in clinical application.
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OBJECTIVE:To establishment the method for the determination of content of vitamin A palmitate(VAP) and its related substances by HPLC. METHODS:The HPLC conditions were consisted of Phenomenex Luna C18 column with a mobile phase of a mixture of acetonitrile-isopropanol (90∶10) ,the detection wavelength of 328 nm,the column temperature of 30 ℃ and the flow rate of 1.0 mL?min-1. RESULTS:VAP was completely separated from impurities,the linearity range was 90～400 mg?L-1(r=0.999 2). The average recovery rate was 99.60% (RSD=1.32%). The average content of the related substances were lower than 2.53% . CONCLUSION: This accurate and reliable HPLC method is applicable for the quality control of VAP.
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OBJECTIVE: To establish a HPLC method for the simultaneously determination of Vitamin D2, Vitamin A and Vitamin E in Cernevit-12 for injection. METHODS: An Inertsil(ODS-2) C18 column was selected as separation column at 30℃. The mobile phase consisted of acetonitrile-methanol-methylene chloride (80∶10∶10) at a flow rate of 1.0mL?min-1. The detection wavelength was set at 265nm and the injection volume was 10?L. RESULTS: The linear ranges of Vitamin D2, Vitamin E acetic ester and Vitamin A palmitate were 0.61～1.43?g?mL-1, 222.6～519.4?g?mL-1 and 1.21～2.82mg?mL-1 respectively. The average recoveries were 99.79%, 99.53% and 101.63%, with RSD of 1.66%, 0.184 6%, 0.113 4%. CONCLUSION: The method is simple, rapid and accurate, and suitable for the determination of Cernevit-12.